|
KMID : 0545120150250050658
|
|
Journal of Microbiology and Biotechnology
2015 Volume.25 No. 5 p.658 ~ p.661
|
|
|
Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis
|
|
Gopal Prasad Ghimire
Niranjan Koirala
Sohng Jae-Kyung
|
|
|
Abstract
|
|
|
|
Genes encoding enzymes with sequence similarity to hopanoids biosynthetic enzymes of other organisms were cloned from the hopanoid (hop) gene cluster of Streptomyces peucetius ATCC 27952 and transformed into Streptomyces venezuelae YJ028. The cloned fragments contained four genes, all transcribed in one direction. These genes encode polypeptides that resemble polyprenyl diphosphate synthase (hopD), squalene-phytoene synthases (hopAB), and squalenehopene cyclase (hopE). These enzymes are sufficient for the formation of the pentacyclic triterpenoid lipid, hopene. The formation of hopene was verified by gas chromatography/ mass spectrometry.
|
|
|
KEYWORD
|
|
|
Cryptic genes, Genome, Heterologous expression, Hopanoid biosynthesis, S. peucetius
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|